DNA Vaccines pp 131-140 | Cite as

Intradermal Vaccination by DNA Tattooing

  • Joost H. van den Berg
  • Koen Oosterhuis
  • Ton N. M. Schumacher
  • John B. A. G. Haanen
  • Adriaan D. BinsEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1143)


DNA vaccination is an attractive vaccination method. First, the production of plasmid DNA as a vaccine is considerably more cheap and simple than the production of recombinant protein. Second, the expression cassette of DNA vaccines can readily be modified, making DNA vaccines highly flexible. Finally, in animal models, DNA vaccination is able to induce potent cellular immune responses.

Over the past decade, the focus in the DNA vaccination field has in large part moved from intramuscular immunization towards dermal administration. As a natural “porte d’entrée” for pathogens, the skin is rich in antigen-presenting cells, which are required for generating an efficient antigen-specific immune response.

This chapter describes a DNA vaccination protocol that utilizes a simple tattooing device for the dermal delivery of plasmid DNA. This technique, called DNA tattooing, is capable of generating high frequencies of antigen-reactive T cells in mice and macaques.

Key words

DNA vaccination Tattooing Intradermal delivery Immune response Plasmid DNA 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Joost H. van den Berg
    • 1
  • Koen Oosterhuis
    • 1
  • Ton N. M. Schumacher
    • 1
  • John B. A. G. Haanen
    • 1
  • Adriaan D. Bins
    • 1
    Email author
  1. 1.Division of ImmunologyThe Netherlands Cancer InstituteAmsterdamThe Netherlands

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