DNA Vaccines pp 159-179 | Cite as

Multivalent DNA-Based Vectors for DNA Vaccine Delivery

  • Young Hoon Roh
  • Kwang Lee
  • Jessica Jane Ye
  • Dan Luo
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1143)

Abstract

DNA can be utilized as a generic delivery vector as well as a traditional biological material for DNA vaccination. Although the use of DNA as an antigen expression vector or a vaccine adjuvant has been intensively studied for several decades, the use of DNA molecules as a delivery carrier has not been explored until recently. This issue is probably due to the topological limitation of DNA in its natural linear or circular structure form. Multivalent DNA-based vector delivery platforms overcome this structural barrier and are particularly suited for DNA vaccine delivery because of their multifunctionality, monodispersity, anisotropicity, and bioconjugation ability with numerous functional moieties. In this chapter, we mainly describe the construction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.

Key words

DNA Dendrimer-like DNA Drug delivery Anisotropicity Multifunctionality DNA engineering DNA vaccines 

References

  1. 1.
    Luo D, Saltzman WM (2000) Synthetic DNA delivery systems. Nat Biotechnol 18:33–37PubMedCrossRefGoogle Scholar
  2. 2.
    Luo D (2003) The road from biology to materials. Mater Today 6:38–43CrossRefGoogle Scholar
  3. 3.
    Lee JB, Roh YH, Um SH et al (2009) Multifunctional nanoarchitectures from DNA-based ABC monomers. Nat Biotechnol 4:430–436Google Scholar
  4. 4.
    Li Y, Tseng YD, Kwon SY et al (2004) Controlled assembly of dendrimer-like DNA. Nat Mater 3:38–42PubMedCrossRefGoogle Scholar
  5. 5.
    Roh YH, Lee JB, Tan SJ et al (2010) Photocrosslinked DNA nanospheres for drug delivery. Macromol Rapid Commun 31: 1207–1211PubMedCrossRefGoogle Scholar
  6. 6.
    Roh YH, Lee JB, Kiatwuthinon P et al (2011) DNAsomes: multifunctional DNA-based nanocarriers. Small 7:74–78PubMedCrossRefGoogle Scholar
  7. 7.
    Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3015PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
  9. 9.
    Roh YH, Ruiz RCH, Peng S et al (2011) Engineering DNA-based functional materials. Chem Soc Rev 40:5730–5744PubMedCrossRefGoogle Scholar
  10. 10.
    Park N, Kahn J, Rice EJ et al (2009) High-yield cell-free protein production from P-gel. Nat Protoc 4:1759–1770PubMedCrossRefGoogle Scholar
  11. 11.
    Um SH, Lee JB, Kwon SY et al (2006) Dendrimer-like DNA-based fluorescence nanobarcodes. Nat Protoc 1:995–1000PubMedCrossRefGoogle Scholar
  12. 12.
    Roh YH, Park JH, Ye JJ et al (2012) Systematic studies of UV stability and photopolymerization efficiency of DNA-based nanomaterials. ChemPhysChem 13:2517–2521PubMedCrossRefGoogle Scholar
  13. 13.
    Zanta MA, Belguise-Valladier P, Behr JP (1999) Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus. Proc Natl Acad Sci U S A 96:91–96PubMedCentralPubMedCrossRefGoogle Scholar
  14. 14.
    Bonny C, Oberson A, Negri S et al (2001) Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death. Diabetes 50:77–82PubMedCrossRefGoogle Scholar
  15. 15.
    Keller M, Tagawa T, Preuss M et al (2002) Biophysical characterization of the DNA binding and condensing properties of adenoviral core peptide mu. Biochemistry 41:652–659PubMedCrossRefGoogle Scholar
  16. 16.
    Corbel SY, Rossi FM (2002) Latest developments and in vivo use of the Tet system: ex vivo and in vivo delivery of tetracycline-regulated genes. Curr Opin Biotechnol 13:448–452PubMedCrossRefGoogle Scholar
  17. 17.
    Mohri K, Nishikawa M, Takahashi N et al (2012) Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells. ACS Nano 6:5931–5940PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Young Hoon Roh
    • 1
  • Kwang Lee
    • 2
  • Jessica Jane Ye
    • 3
  • Dan Luo
    • 2
  1. 1.David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of Biological and Environmental EngineeringCornell UniversityIthacaUSA
  3. 3.School of Medicine, Yale UniversityNew HavenUSA

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