DNA Vaccines

Volume 1143 of the series Methods in Molecular Biology pp 159-179


Multivalent DNA-Based Vectors for DNA Vaccine Delivery

  • Young Hoon RohAffiliated withDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology Email author 
  • , Kwang LeeAffiliated withDepartment of Biological and Environmental Engineering, Cornell University
  • , Jessica Jane YeAffiliated withSchool of Medicine, Yale University
  • , Dan LuoAffiliated withDepartment of Biological and Environmental Engineering, Cornell University

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DNA can be utilized as a generic delivery vector as well as a traditional biological material for DNA vaccination. Although the use of DNA as an antigen expression vector or a vaccine adjuvant has been intensively studied for several decades, the use of DNA molecules as a delivery carrier has not been explored until recently. This issue is probably due to the topological limitation of DNA in its natural linear or circular structure form. Multivalent DNA-based vector delivery platforms overcome this structural barrier and are particularly suited for DNA vaccine delivery because of their multifunctionality, monodispersity, anisotropicity, and bioconjugation ability with numerous functional moieties. In this chapter, we mainly describe the construction of multivalent DNA-based delivery vectors using DNA engineering methods. Specifically, the synthesis strategies for highly branched dendrimer-like DNA structures in general and methods for their application to DNA vaccine delivery are introduced.

Key words

DNA Dendrimer-like DNA Drug delivery Anisotropicity Multifunctionality DNA engineering DNA vaccines