Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
Key wordsLipid nanoparticle Microfluidics siRNA Nanoparticle manufacture Solid-core LNP siRNA-LNP NanoAssemblr Nanoparticle formulation
This work was supported by the National Science and Engineering Research Council of Canada (F09-04486), Canadian Institutes for Health Research (111627), and Genome British Columbia.
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