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General In Vitro Caspase Assay Procedures

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Caspases,Paracaspases, and Metacaspases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1133))

Abstract

One of the most valuable tools that have been developed for the study of apoptosis is the availability of recombinant active caspases. The determination of caspase substrate preference, the design of sensitive substrates and potent inhibitors, the resolution of caspase structures, the elucidation of their activation mechanisms, and the identification of their substrates were made possible by the availability of sufficient amounts of enzymatically pure caspases. The current chapter describes at length the expression, purification, and basic enzymatic characterization of apoptotic caspases.

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Author information

Authors and Affiliations

  1. Institute of Molecular Bioscience, University of Queensland, St. Lucia, QLD, Australia

    Dave Boucher

  2. Department of Pharmacology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada

    Catherine Duclos & Jean-Bernard Denault

Authors
  1. Dave Boucher
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  2. Catherine Duclos
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  3. Jean-Bernard Denault
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Editor information

Editors and Affiliations

  1. Dept of Plant Biology & Forest Genetics, Swedish Univ of Agricultural Sciences Linnean Center for Plant Biology, Uppsala, Sweden

    Peter V. Bozhkov

  2. Sanford-Burnham Medical Research Inst. Apoptosis & Cell Death Research Prgm, La Jolla, California, USA

    Guy Salvesen

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Boucher, D., Duclos, C., Denault, JB. (2014). General In Vitro Caspase Assay Procedures. In: V. Bozhkov, P., Salvesen, G. (eds) Caspases,Paracaspases, and Metacaspases. Methods in Molecular Biology, vol 1133. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0357-3_1

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  • DOI: https://doi.org/10.1007/978-1-4939-0357-3_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0356-6

  • Online ISBN: 978-1-4939-0357-3

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