Screening Proteins for NMR Suitability
NMR spectroscopy is a valuable tool in structural genomics. Identification of protein samples that are amenable to structure determination by NMR spectroscopy requires efficient screening. The preparation of multiple samples in parallel and screening by NMR is described. The method described is applicable to large structural genomics projects but can easily be scaled down for application to small structural biology projects. All the equipment used is commonly found in any NMR structural biology laboratory.
Key wordsNMR screening Autoinduction Structural genomics NMR spectroscopy NMR sample
We acknowledge the support of NIGMS Protein Structure Initiative grant U54-GM094597 to NESG consortium, the Natural Sciences and Engineering Research Council of canada and the Canada Foundation for Innovation for infrastructure funding.
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