Laser Doppler Flowmetry to Measure Changes in Cerebral Blood Flow

  • Brad A. Sutherland
  • Tamer Rabie
  • Alastair M. Buchan
Part of the Methods in Molecular Biology book series (MIMB, volume 1135)

Abstract

Laser Doppler flowmetry (LDF) is a method by which relative cerebral blood flow (CBF) of the cortex can be measured. Although the method is easy to employ, LDF only measures relative CBF, while absolute CBF cannot be quantified. LDF is useful for investigating CBF changes in a number of different applications including neurovascular and stroke research. This chapter will prepare the reader for rodent experiments using LDF with two preparations. The closed skull preparation can be used to monitor CBF with an intact skull, but in adult rats, thinning of the skull is required to obtain an accurate cortical CBF signal. The open skull preparation requires a craniotomy to expose the surface of the brain and the LDF probe is held close to the surface to measure cerebral perfusion.

Key words

Laser Doppler flowmetry Cerebral blood flow Rodent Relative changes Probe Perfusion 

References

  1. 1.
    Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with iodo [14c] antipyrine. Am J Physiol 234:H59–H66PubMedGoogle Scholar
  2. 2.
    Bonner R, Nossal R (1981) Model for laser doppler measurements of blood flow in tissue. Appl Opt 20:2097–2107CrossRefPubMedGoogle Scholar
  3. 3.
    Stern MD (1975) In vivo evaluation of microcirculation by coherent light scattering. Nature 254:56–58CrossRefPubMedGoogle Scholar
  4. 4.
    Dirnagl U, Kaplan B, Jacewicz M, Pulsinelli W (1989) Continuous measurement of cerebral cortical blood flow by laser-doppler flowmetry in a rat stroke model. J Cereb Blood Flow Metab 9:589–596CrossRefPubMedGoogle Scholar
  5. 5.
    Kiel JW, Riedel GL, DiResta GR, Shepherd AP (1985) Gastric mucosal blood flow measured by laser-doppler velocimetry. Am J Physiol 249:G539–G545PubMedGoogle Scholar
  6. 6.
    Stern MD, Bowen PD, Parma R, Osgood RW, Bowman RL, Stein JH (1979) Measurement of renal cortical and medullary blood flow by laser-doppler spectroscopy in the rat. Am J Physiol 236:F80–F87PubMedGoogle Scholar
  7. 7.
    Sundqvist T, Oberg PA, Rapoport SI (1985) Blood flow in rat sciatic nerve during hypotension. Exp Neurol 90:139–148CrossRefPubMedGoogle Scholar
  8. 8.
    Jakobsson A, Nilsson GE (1993) Prediction of sampling depth and photon pathlength in laser doppler flowmetry. Med Biol Eng Comput 31:301–307CrossRefPubMedGoogle Scholar
  9. 9.
    Chen RL, Nagel S, Papadakis M, Bishop T, Pollard P, Ratcliffe PJ et al (2012) Roles of individual prolyl-4-hydroxylase isoforms in the first 24 hours following transient focal cerebral ischaemia: insights from genetically modified mice. J Physiol 590:4079–4091CrossRefPubMedCentralPubMedGoogle Scholar
  10. 10.
    Piilgaard H, Lauritzen M (2009) Persistent increase in oxygen consumption and impaired neurovascular coupling after spreading depression in rat neocortex. J Cereb Blood Flow Metab 29:1517–1527CrossRefPubMedGoogle Scholar
  11. 11.
    Attwell D, Buchan AM, Charpak S, Lauritzen M, Macvicar BA, Newman EA (2010) Glial and neuronal control of brain blood flow. Nature 468:232–243CrossRefPubMedCentralPubMedGoogle Scholar
  12. 12.
    Lecrux C, Toussay X, Kocharyan A, Fernandes P, Neupane S, Levesque M et al (2011) Pyramidal neurons are “neurogenic hubs” in the neurovascular coupling response to whisker stimulation. J Neurosci 31:9836–9847CrossRefPubMedGoogle Scholar
  13. 13.
    Mathiesen C, Caesar K, Thomsen K, Hoogland TM, Witgen BM, Brazhe A et al (2011) Activity-dependent increases in local oxygen consumption correlate with postsynaptic currents in the mouse cerebellum in vivo. J Neurosci 31:18327–18337CrossRefPubMedGoogle Scholar
  14. 14.
    Paxinos G, Watson C (1997) The rat brain in stereotaxic coordinates, 3rd edn. Academic, San Diego, CAGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Brad A. Sutherland
    • 1
  • Tamer Rabie
    • 1
  • Alastair M. Buchan
    • 2
  1. 1.Radcliffe Department of MedicineUniversity of OxfordOxfordUK
  2. 2.Acute Stroke Programme, Radcliffe Department of MedicineUniversity of OxfordOxfordUK

Personalised recommendations