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Live-Imaging of the Arabidopsis Inflorescence Meristem

  • Marcus G. Heisler
  • Carolyn Ohno
Part of the Methods in Molecular Biology book series (MIMB, volume 1110)

Abstract

The aboveground tissues of higher plants are derived from a small population of stem cells located at the shoot apex within a structure called the shoot apical meristem (SAM). The SAM not only includes the stem cells but also incorporates a region from which lateral organs arise. The SAM is therefore of prime interest for understanding plant growth and development. In this chapter we outline methods for using confocal microscopy to image the Arabidopsis inflorescence SAM. This method enables detailed examination of cell division and growth patterns (Reddy et al., Development 131:4225–4237, 2004) as well as gene expression and protein localization patterns over time (Heisler et al. Curr Biol 15:1899–1911, 2005). When combined with perturbation approaches, the method offers an extremely powerful system for investigating SAM function in great detail.

Keywords

Arabidopsis Confocal Imaging Shoot meristem Flowers 

Notes

Acknowledgements

We acknowledge the Australian Research Council and European Research Council for support.

References

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Developmental Biology UnitEuropean Molecular Biology LaboratoryHeidelbergGermany
  2. 2.School of Biological SciencesUniversity of SydneySydneyAustralia

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