Next-Generation Sequencing Applied to Flower Development: ChIP-Seq

  • Emmanuelle GracietEmail author
  • Diarmuid Seosamh Ó’Maoiléidigh
  • Frank WellmerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1110)


Over the past 20 years, classic genetic approaches have shown that the developmental program underlying flower formation involves a large number of transcriptional regulators. However, the target genes of these transcription factors, as well as the gene regulatory networks they control, remain largely unknown. Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-Seq), which allows the identification of transcription factor binding sites on a genome-wide scale, has been successfully applied to a number of transcription factors in Arabidopsis. The ChIP-Seq procedure involves chemical cross-linking of proteins to DNA, followed by chromatin fragmentation and immunoprecipitation of specific protein–DNA complexes. The regions of the genome bound by a specific transcription factor can then be identified after next-generation sequencing.


Arabidopsis Flower development ChIP-Seq Transcription factors 



Work in our laboratory is funded by grants from Science Foundation Ireland to F.W. and E.G.


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Smurfit Institute of GeneticsTrinity CollegeDublinIreland
  2. 2.Smurfit Institute of GeneticsTrinity College DublinDublinIreland

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