Protocol

Flower Development

Volume 1110 of the series Methods in Molecular Biology pp 401-411

Date:

Next-Generation Sequencing Applied to Flower Development: RNA-Seq

  • Jun HeAffiliated withState Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
  • , Yuling JiaoAffiliated withState Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Email author 

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Abstract

Genome-wide study of gene expression, or transcriptome profiling, is critical for our understanding of biological functions, including developmental processes. Recent breakthroughs in high-throughput sequencing technologies have revolutionized gene expression profiling to study the transcriptome at the nucleotide level, which is known as RNA-seq. RNA-seq, also called “whole transcriptome shotgun sequencing,” uses next-generation sequencing technologies to sequence cDNA in order to infer a sample’s RNA content. Here we describe a detailed bench-ready protocol to generate RNA-seq libraries for high-throughput single-end or pair-end sequencing compatible with the Illumina sequencing platform.

Keywords

Next-generation sequencing RNA-seq Transcriptome