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Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

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Mitosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2415))

Abstract

The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the absence of transgenic lines, alternative strategies must be employed to visualize microtubules and actin. Here, we describe methods to visualize actin and microtubule using either purified, recombinant proteins, or probes in in vitro-transcribed mRNAs.

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Acknowledgements

The authors want to thank David Burgess (Boston College) for the Lifeact-GFP plasmid. This work was supported by the National Science Foundation to C.B.S (MCB-1917983) and an American Cancer Society postdoctoral fellowship to S.Z.S. (PF-16-007-01-CCG).

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Authors and Affiliations

  1. Department of Biology, New Mexico State University, Las Cruces, NM, USA

    Debadrita Pal, Florencia Visconti, Silvia P. Sepúlveda-Ramírez & Charles B. Shuster

  2. Whitehead Institute for Biomedical Research, Cambridge, MA, USA

    S. Zachary Swartz

Authors
  1. Debadrita Pal
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  2. Florencia Visconti
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  3. Silvia P. Sepúlveda-Ramírez
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  4. S. Zachary Swartz
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  5. Charles B. Shuster
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Corresponding author

Correspondence to Charles B. Shuster .

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Editors and Affiliations

  1. The Hormel Institute, University of Minnesota, Austin, MN, USA

    Edward H. Hinchcliffe

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Pal, D., Visconti, F., Sepúlveda-Ramírez, S.P., Swartz, S.Z., Shuster, C.B. (2022). Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis. In: Hinchcliffe, E.H. (eds) Mitosis. Methods in Molecular Biology, vol 2415. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1904-9_1

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  • DOI: https://doi.org/10.1007/978-1-0716-1904-9_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1903-2

  • Online ISBN: 978-1-0716-1904-9

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