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Proteomics for Development of Food Allergy Vaccines

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Book cover Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2410))

Abstract

Food allergy is a hypersensitivity reaction to food products initiated by immunologic mechanisms, which represents one of the major concerns in food safety. New therapies for food allergies including oral and epicutaneous allergen-specific immunotherapy are required, and B cell epitope-based allergy vaccines are a good promise to improve this field. In this chapter, we describe a workflow for the design of food allergy vaccines using proteomic tools. The strategy is defined based on the characterization of B cell epitopes for a particular food allergen. For that, the workflow comprises five consecutive steps: (1) shotgun proteomics analysis of different protein isoforms for a particular food allergen, (2) downloading all protein sequences for the specific allergen included in UniProtKB database, (3) analysis by protein-based bioinformatics of B cell epitopes, (4) synthesizing of the selected B cell peptide epitopes, and (5) performing of immunoassays using sera from healthy and allergic patients. The results from this method provide a rationale repository of B cell epitopes for the design of new specific immunotherapies for a particular food allergen. The strategy was optimized for all the beta-parvalbumins (β-PRVBs), which are considered as the main fish allergens. Using this workflow, a total of 35 peptides were identified as B cell epitopes, among them the top 4 B cell peptide epitopes that may induce protective immune response were selected as potential peptide vaccine candidates for fish allergy.

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Acknowledgments

This work was supported by the GAIN-Xunta de Galicia Project (IN607D 2017/01) and the Spanish AEI/EU-FEDER PID2019-103845RB-C21 project. Dr. Mónica Carrera is supported by the Ramón y Cajal contract (Ministry of Science and Innovation of Spain). Dr. Susana Magadan is supported by the Talent Retention program of Universidade de Vigo.

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Authors and Affiliations

  1. Department of Food Technology, Spanish National Research Council (CSIC), Institute of Marine Research (IIM), Pontevedra, Spain

    Mónica Carrera

  2. Biomedical Research Center (CINBIO), Universidade de Vigo, Immunology, Pontevedra, Spain

    Susana Magadán

Authors
  1. Mónica Carrera
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  2. Susana Magadán
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Corresponding author

Correspondence to Susana Magadán .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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Carrera, M., Magadán, S. (2022). Proteomics for Development of Food Allergy Vaccines. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2410. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1884-4_36

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  • DOI: https://doi.org/10.1007/978-1-0716-1884-4_36

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1883-7

  • Online ISBN: 978-1-0716-1884-4

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