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Cell Penetrating Peptides pp 385–412Cite as

New Tools for Streamlined In Vivo Homing Peptide Identification

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2383))

Abstract

In vivo peptide-phage display is an unbiased technique for mapping of the vascular diversity and identification of homing peptides. This chapter is intended to serve as a structured practical guide to execute in vivo T7 phage biopanning and data analysis experiments. We discuss experimental designs and protocols with emphasis on application of high-throughput sequencing-based technologies for streamlined in vivo biopanning and validation of homing peptides.

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Acknowledgments

This work was supported by the European Regional Development Fund (Project No. 2014-2020.4.01.15-0012), by EMBO Installation grant #2344 (to T. Teesalu), European Research Council grant GLIOGUIDE (780915) from European Regional Development Fund (to T. Teesalu), Wellcome Trust International Fellowship WT095077MA (to T. Teesalu), and Norwegian-Estonian collaboration grant EMP181 (to T. Teesalu). We also acknowledge the support of Estonian Research Council (grants PRG230 and EAG79 to T. Teesalu). K. Pleiko was supported by a PhD research scholarship administered by the University of Latvia Foundation and funded by “Mikrotikls” Ltd.

Figures 1 and 3 were created using BioRender.com.

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Author notes

  1. Kristina Põšnograjeva and Karlis Pleiko contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia

    Kristina Põšnograjeva, Karlis Pleiko, Maarja Haugas & Tambet Teesalu

  2. Faculty of Medicine, University of Latvia, Riga, Latvia

    Karlis Pleiko

  3. Cancer Research Center, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA

    Tambet Teesalu

  4. Center for Nanomedicine and Department of Cell, Molecular and Developmental Biology, University of California, Santa Barbara, CA, USA

    Tambet Teesalu

Authors
  1. Kristina Põšnograjeva
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  2. Karlis Pleiko
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  3. Maarja Haugas
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  4. Tambet Teesalu
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Corresponding author

Correspondence to Tambet Teesalu .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Põšnograjeva, K., Pleiko, K., Haugas, M., Teesalu, T. (2022). New Tools for Streamlined In Vivo Homing Peptide Identification. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_25

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  • DOI: https://doi.org/10.1007/978-1-0716-1752-6_25

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1751-9

  • Online ISBN: 978-1-0716-1752-6

  • eBook Packages: Springer Protocols

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