Abstract
Phragmoplasts are plant-specific microtubule structures that form cell plates at the cell division plane. During late anaphase, phragmoplasts emerge between daughter nuclei as the derivative of spindle microtubules, and centrifugally expand toward the cell cortex to build cell plates during telophase. Phragmoplasts are composed of short antiparallel microtubules decorated with various microtubule-associated proteins. Mutants of these microtubule-associated proteins exhibit defects in phragmoplast morphology. Quantification of phragmoplast morphology is indispensable for assessing the phenotypes of these mutants. Here, we describe a method to quantify the width of phragmoplasts.
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Acknowledgments
This work was supported by grants from JSPS KAKENHI (19H05677 to YO and 18K14737 and 19Â J01358 to TS).
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Sasaki, T., Oda, Y. (2022). A Quantitative Method for Evaluating Phragmoplast Morphology. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 2382. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1744-1_13
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DOI: https://doi.org/10.1007/978-1-0716-1744-1_13
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