Abstract
Interface tissues are functionally graded tissues characterized by a complex layered structure, which therefore present a great challenge to be reproduced and cultured in vitro. Here, we describe the design and operation of a 3D printed dual-chamber bioreactor as a culturing system for biphasic native or engineered osteochondral tissues. The bioreactor is designed to potentially accommodate a variety of interface tissues and enables the precise study of tissue crosstalk by creating two separate microenvironments while maintaining the tissue compartments in direct contact.
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Acknowledgments
The authors acknowledge current and former members of the Center for Cellular and Molecular Engineering at the University of Pittsburgh who contributed to the design of the bioreactor and the development of protocols, including Dr. Rocky S. Tuan, Dr. Hang Lin, Dr. Peter G. Alexander, Dr. Thomas P. Lozito, Dr. Gioacchino Conoscenti, and Dr. Alessandro Pirosa. The authors acknowledge the contributions of Mr. Andy Holmes at the Swanson Center for Product Innovation at the University of Pittsburgh to the fabrication of the bioreactor. The work leading to the development of the bioreactor and of these protocols was supported by grants from the Commonwealth of Pennsylvania Department of Health, the National Institutes of Health (1U18 TR000532-01), the Ri.MED Foundation, the Children’s Hospital of Philadelphia Research Institute, and the Frontier Program in Airway Disorders of the Children’s Hospital of Philadelphia.
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Chiesa, I., Di Gesù, R., Overholt, K.J., Gottardi, R. (2022). A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit. In: Rasponi, M. (eds) Organ-on-a-Chip. Methods in Molecular Biology, vol 2373. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1693-2_16
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DOI: https://doi.org/10.1007/978-1-0716-1693-2_16
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Online ISBN: 978-1-0716-1693-2
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