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Antimicrobial Therapies pp 393–408Cite as

Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi)

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Part of the Methods in Molecular Biology book series (MIMB,volume 2296)

Abstract

The human disease tuberculosis (TB) is the leading cause of death from a single infectious agent. A quarter of the world’s population is estimated to be latently infected. Drug development and screening is slow and costly. We have developed a physiologically relevant assay to screen drugs against TB when inside immune cells. This chapter will describe a newly developed preclinical drug screening assay for TB, using high-content imaging and pharmacokinetic/pharmacodynamic modeling.

Key words

  • Tuberculosis
  • Drug screening
  • Mycobacteria
  • Antibiotics
  • Pharmacokinetic and pharmacodynamic drug Modeling

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  • DOI: 10.1007/978-1-0716-1358-0_23
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Author information

Authors and Affiliations

  1. Centre for Drugs and Diagnostics, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, UK

    Samantha Donnellan, Ghaith Aljayyoussi & Giancarlo A. Biagini

  2. Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, The University of Liverpool, Liverpool, UK

    Carmen Martínez-Rodríguez

Authors
  1. Samantha Donnellan
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  2. Carmen Martínez-Rodríguez
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  3. Ghaith Aljayyoussi
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  4. Giancarlo A. Biagini
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Corresponding author

Correspondence to Samantha Donnellan .

Editor information

Editors and Affiliations

  1. INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma A Subsidiary of Albany Molecular Research Inc. (AMRI) Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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Donnellan, S., Martínez-Rodríguez, C., Aljayyoussi, G., Biagini, G.A. (2021). Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose–Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi). In: Barreiro, C., Barredo, JL. (eds) Antimicrobial Therapies. Methods in Molecular Biology, vol 2296. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1358-0_23

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  • DOI: https://doi.org/10.1007/978-1-0716-1358-0_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1357-3

  • Online ISBN: 978-1-0716-1358-0

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