In Vivo Administration of Therapeutic Antisense Oligonucleotides

  • Luisa Statello
  • Mohamad Moustafa Ali
  • Chandrasekhar KanduriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2254)


With the rapid revolution in RNA/DNA sequencing technologies, it is evident that mammalian genomes express tens of thousands of long noncoding RNAs (lncRNAs). Since a large majority of lncRNAs have been functionally implicated in cancer development and progression, there is an increasing appreciation for the use of antisense oligonucleotide (ASO)-based therapies targeting lncRNAs in several cancers. Despite their great potential in therapeutic applications, their use is still limited due to cellular toxicity and shortcomings in achieving required stability in biological fluids and tissue uptake. To overcome these limitations, major changes in ASO chemistry have been introduced to generate second and third generation ASOs, including locked nucleic acids (LNA) technology. Here we describe two different LNA-ASO delivery approaches, a peritumoral administration and a systemic delivery in xenograft models of lung adenocarcinoma, that significantly reduced tumor growth without inducing toxicity.

Key words

Antisense oligonucleotides LNA-ASOs Xenografts Lung cancer lncRNAs Long noncoding RNAs 



This work was supported by the grants from Knut and Alice Wallenberg Foundation [KAW2014.0057], Swedish Foundation for Strategic Research [RB13-0204], Swedish Cancer Research foundation [Cancerfonden: Kontrakt no. CAN2018/591], Swedish Research Council [2017-02834], Barncancerfonden [PR2018-0090], Ingabritt Och Arne Lundbergs forskningsstiftelse, and LUA/ALF (to C.K.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  • Luisa Statello
    • 1
  • Mohamad Moustafa Ali
    • 1
  • Chandrasekhar Kanduri
    • 1
    Email author
  1. 1.Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden

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