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Plant Transposable Elements pp 195–205Cite as

Measurement of Genetic Mobility Using a Transposon-Based Marker System in Sorghum

Part of the Methods in Molecular Biology book series (MIMB,volume 2250)

Abstract

Transposable elements (TEs) are ubiquitous repetitive components of eukaryotic organisms that show mobility in the genome against diverse stresses. TEs contribute considerably to the size, structure, and plasticity of genomes and also play an active role in genome evolution by helping their hosts adapt to novel conditions by conferring useful characteristics. We developed a simple and rapid method for investigation of genetic mobility and diversity among TEs in combination with a target region amplification polymorphism (TE-TRAP) marker system in gamma-irradiated sorghum mutants. The TE-TRAP marker system reveals a high level of genetic diversity, which provides a useful marker resource for genetic mobility research.

Key words

  • TE-TRAP
  • Transposable elements
  • Sorghum bicolor
  • Genetic diversity
  • PCR-based marker

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Acknowledgment

This work was supported by the research program of KAERI, Republic of Korea.

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Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, South Korea

    Jae Il Lyu, Yeong Deuk Jo, Joon-Woo Ahn, Jin-Baek Kim & Soon-Jae Kwon

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  1. Jae Il Lyu
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  2. Yeong Deuk Jo
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  3. Joon-Woo Ahn
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  4. Jin-Baek Kim
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  5. Soon-Jae Kwon
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Corresponding author

Correspondence to Soon-Jae Kwon .

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Editors and Affiliations

  1. CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology & Ecology, Shanghai, China

    Dr. Jungnam Cho

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Lyu, J.I., Jo, Y.D., Ahn, JW., Kim, JB., Kwon, SJ. (2021). Measurement of Genetic Mobility Using a Transposon-Based Marker System in Sorghum. In: Cho, J. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 2250. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1134-0_19

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  • DOI: https://doi.org/10.1007/978-1-0716-1134-0_19

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