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Rapid Detection of Transposon-Induced Genome Rearrangements

Part of the Methods in Molecular Biology book series (MIMB,volume 2250)

Abstract

This chapter details the techniques used to detect transposon-induced genome rearrangements. Here, we describe a rapid DNA isolation technique, PCR amplification, and a novel High Efficiency Agarose Gel Electrophoresis Method (HEA-GEM).

Key words

  • Rapid DNA isolation
  • Plate DNA extraction
  • PCR
  • High efficiency agarose gel electrophoresis
  • Large population size

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  • DOI: 10.1007/978-1-0716-1134-0_13
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Acknowledgments

This research is supported by the USDA National Institute of Food and Agriculture Hatch project number IOW05282, and by State of Iowa funds.

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Correspondence to Thomas Peterson .

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Sharma, S.P., Peterson, T. (2021). Rapid Detection of Transposon-Induced Genome Rearrangements. In: Cho, J. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 2250. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1134-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1134-0_13

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