EPR Spectroscopy as a Method for ROS Quantification in the Skin
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Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.
KeywordsFree radicals Electron paramagnetic resonance (EPR) spectroscopy UVA light Spin probe Skin Cumulative radical production
This work was funded by the German Federal Ministry of Education and Research BMBF (Project number 03ZZ0140A) within the program “Twenty20—Partnership for Innovation” and carried out within the consortium “Advanced UV for Life.”
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