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EPR Spectroscopy as a Method for ROS Quantification in the Skin

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2202)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.

Keywords

Free radicals Electron paramagnetic resonance (EPR) spectroscopy UVA light Spin probe Skin Cumulative radical production 

Notes

Acknowledgments

This work was funded by the German Federal Ministry of Education and Research BMBF (Project number 03ZZ0140A) within the program “Twenty20—Partnership for Innovation” and carried out within the consortium “Advanced UV for Life.”

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous PhysiologyBerlinGermany
  2. 2.Freiberg Instruments GmbHFreibergGermany

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