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Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components

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Lipid Rafts

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2187))

Abstract

Communication between cells and their environment is carried out through the plasma membrane including the action of most pharmaceutical drugs. Although such a communication typically involves specific binding of a messenger to a membrane receptor, the biophysical state of the lipid bilayer strongly influences the outcome of this interaction. Sphingolipids constitute an important part of the lipid membrane, and their mole fraction modifies the biophysical characteristics of the membrane. Here, we describe methods that can be used for measuring how sphingolipid accumulation alters the compactness, microviscosity, and dipole potential of the lipid bilayer and the mobility of membrane components.

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Acknowledgments

This work was supported by research grants from the National Research, Development and Innovation Office, Hungary (K120302, GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00044, EFOP-3.6.3-VEKOP-16-2017-00009).

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Authors and Affiliations

  1. Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Gyula Batta, Tímea Hajdu & Peter Nagy

  2. Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary

    Gyula Batta

  3. Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Tímea Hajdu

Authors
  1. Gyula Batta
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  2. Tímea Hajdu
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  3. Peter Nagy
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Corresponding author

Correspondence to Peter Nagy .

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Editors and Affiliations

  1. Department of Physiology, University of Kentucky Medical School, Lexington, KY, USA

    Erhard Bieberich

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Batta, G., Hajdu, T., Nagy, P. (2021). Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components. In: Bieberich, E. (eds) Lipid Rafts. Methods in Molecular Biology, vol 2187. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0814-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0814-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0813-5

  • Online ISBN: 978-1-0716-0814-2

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