Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D).
Cryo-TEM Plunge freezing Electron Tomography Immunogold labeling Vaccine delivery carriers
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This work was supported by the Nanotechnology Research Center and the Human Health Therapeutics Research Center at the National Research Council of Canada.
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