Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs

  • Quentin R. V. FerryEmail author
  • Tudor A. Fulga
Part of the Methods in Molecular Biology book series (MIMB, volume 2162)


The type-II CRISPR-Cas9 system has been repurposed to create synthetic programmable transcriptional regulators (CRISPR-TRs). Subsequent modifications of the system now allow for spatiotemporal control of CRISPR-mediated gene activation and repression. Among these solutions, the development of inducible spacer-blocking hairpin guide RNAs (iSBH-sgRNAs) provide an easy to implement and versatile way to condition the activation of most CRISPR-TRs on the presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.

Key words

CRISPR Cas9 Inducible sgRNA Transcription 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  1. 1.Radcliffe Department of Medicine, Weatherall Institute of Molecular MedicineUniversity of OxfordOxfordUK

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