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Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells

  • Francisco Hernández-Torres
  • Lara Rodríguez-Outeiriño
  • Amelia AránegaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2155)

Abstract

It has been shown that freshly isolated satellite cells from adult muscle constitute a stem cell-like population that exhibits more efficient engraftment and self-renewal activity in regenerating muscle than myoblast. Thus, purification of pure populations of quiescent satellite cells from adult skeletal muscle is highly necessary, not only for understanding the biology of satellite cells and myoblasts but also for improving cell-based therapies for muscle regeneration. This chapter describes a basic protocol used in our laboratory to isolate quiescent muscle satellite cells from adult skeletal muscle by enzymatic dissociation followed by a sequential magnetic-activated cell sorting (MACS). This method is cheap and fast providing and alternative procedure to other purification methods that require fluorescence-activated cell sorting (FACS) machines. Freshly isolated quiescent satellite cells purified by this method can be used in a broad range of experiments including cell transplantation for satellite cell self-renewal experiments or cell therapies.

Key words

Skeletal muscle Muscle satellite cells Myogenesis Myogenic differentiation Cell culture Magnetic-activated cell sorting (MACS) cell separation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Francisco Hernández-Torres
    • 1
    • 2
  • Lara Rodríguez-Outeiriño
    • 1
    • 2
  • Amelia Aránega
    • 1
    • 2
    Email author
  1. 1.Faculty of Experimental Sciences, Department of Experimental BiologyUniversity of JaénJaénSpain
  2. 2.Fundación MedinaGranadaSpain

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