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Measuring the Kinase Activity of GCKIII Proteins In Vitro

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Cerebral Cavernous Malformations (CCM)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2152))

Abstract

One of the CCM genes, CCM3/PDCD10, binds to the protein kinase family GCKIII, which comprises MST3/STK24, SOK1/STK25, and MST4/STK26. These proteins have been shown to have the same effect as CCM3, both in endothelial cells and in animal models such as zebrafish and are most likely involved in CCM pathogenesis. We describe here an in vitro kinase assay of GCKIII proteins which can be used to study their regulation in endothelial and other cells under different circumstances.

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Acknowledgments

This work was supported by grants to CMP and JZ from FEDER/Ministerio de Ciencia, Innovación y Universidades-Agencia Estatal de Investigación of Spain (SAF2017-87691-R), and Xunta de Galicia (ED431C 2019/13). MS is a predoctoral fellow from Xunta de Galicia.

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Author notes

  1. Cristina Almengló

    Present address: Instituto de Investigación Sanitaria, Complexo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain

Authors and Affiliations

  1. Departamento de Fisiología and CiMUS, Universidade de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain

    Juan Zalvide, Cristina Almengló, Sara Vázquez, Mar García-Colomer, Miriam Sartages & Celia M. Pombo

Authors
  1. Juan Zalvide
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  2. Cristina Almengló
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  3. Sara Vázquez
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  4. Mar García-Colomer
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  5. Miriam Sartages
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  6. Celia M. Pombo
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Corresponding authors

Correspondence to Juan Zalvide or Celia M. Pombo .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Chemistry and Pharmacy, University of Siena, SIENA, Italy

    Lorenza Trabalzini

  2. Department of Biotechnology, Chemistry and Pharmacy, University of Siena, SIENA, Italy

    Federica Finetti

  3. Department of Clinical and Biological Sciences, School of Medicine and Surgery, University of Torino, ORBASSANO (TORINO), Italy

    Saverio Francesco Retta

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Zalvide, J., Almengló, C., Vázquez, S., García-Colomer, M., Sartages, M., Pombo, C.M. (2020). Measuring the Kinase Activity of GCKIII Proteins In Vitro. In: Trabalzini, L., Finetti, F., Retta, S. (eds) Cerebral Cavernous Malformations (CCM) . Methods in Molecular Biology, vol 2152. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0640-7_32

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  • DOI: https://doi.org/10.1007/978-1-0716-0640-7_32

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0639-1

  • Online ISBN: 978-1-0716-0640-7

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