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Sphingolipids: Functional and Biological Aspects in Mammals, Plants, and Fungi

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Analysis of Membrane Lipids

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Abstract

Sphingolipids are an important class of lipids found in a variety of organisms, including mammals, plants, and fungi. It is structurally diverse among them, presenting variations in the headgroups as well as in the sites of unsaturation and length of the fatty acid chain. Although structurally different, some molecules are found in mammalian, fungal, and plant cells, such as glycosylceramides. On the other hand, there are sphingolipids only found in certain organisms, such as gangliosides and sphingomyelin in mammals, 9-methyl-4,8-sphingadienine in fungi, and specific inositolphosphorylceramides in plants. A variety of methodologies are available in the literature in order to extract, purify, and identify sphingolipid structures, all of them based on the use of organic solvents, chromatographic, and spectrometric techniques. The study of sphingolipids shows to be necessary when considering the roles for biological events, such as membrane integrity and cell morphology, as well as cellular signaling, nutrient uptake, and regulation of growth. For these reasons, this chapter aims to discuss the most important aspects of sphingolipids that have been studying during the last few decades.

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Authors and Affiliations

  1. Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil

    Rodrigo Rollin-Pinheiro, Mariana Collodetti Bernardino & Eliana Barreto-Bergter

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  1. Rodrigo Rollin-Pinheiro
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  2. Mariana Collodetti Bernardino
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  3. Eliana Barreto-Bergter
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  1. Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Gurgaon, Haryana, India

    Rajendra Prasad

  2. Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, India

    Ashutosh Singh

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Rollin-Pinheiro, R., Bernardino, M.C., Barreto-Bergter, E. (2020). Sphingolipids: Functional and Biological Aspects in Mammals, Plants, and Fungi. In: Prasad, R., Singh, A. (eds) Analysis of Membrane Lipids. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0631-5_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0631-5_3

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