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Photocurable Biopolymers for Coaxial Bioprinting

  • Marco Costantini
  • Andrea Barbetta
  • Wojciech Swieszkowski
  • Dror Seliktar
  • Cesare Gargioli
  • Alberto RainerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2147)

Abstract

Thanks to their unique advantages, additive manufacturing technologies are revolutionizing almost all sectors of the industrial and academic worlds, including tissue engineering and regenerative medicine. In particular, 3D bioprinting is rapidly emerging as a first-choice approach for the fabrication—in one step—of advanced cell-laden hydrogel constructs to be used for in vitro and in vivo studies. This technique consists in the precise deposition layer-by-layer of sub-millimetric hydrogel strands in which living cells are embedded. A key factor of this process consists in the proper formulation of the hydrogel precursor solution, the so-called bioink. Ideal bioinks should be able, on the one side, to support cell growth and differentiation and, on the other, to allow the high-resolution deposition of cell-laden hydrogel strands. The latter feature requires the extruded solution to instantaneously undergo a sol-gel transition to avoid its collapse after deposition.

To address this challenge, researchers are recently focusing their attention on the synthesis of several derivatives of natural biopolymers to enhance their printability. Here, we present an approach for the synthesis of photocurable derivatives of natural biopolymers—namely, gelatin methacrylate, hyaluronic acid methacrylate, chondroitin sulfate methacrylate, and PEGylated fibrinogen—that can be used to formulate tailored innovative bioinks for coaxial-based 3D bioprinting applications.

Key words

Coaxial bioprinting Photocurable polymers Alginate Bioink formulation 

References

  1. 1.
    Gibson I, Rosen D, Stucker B (2015) Additive manufacturing technologies. Springer, New York, NYCrossRefGoogle Scholar
  2. 2.
    Murphy SV, Atala A (2014) 3D bioprinting of tissues and organs. Nat Biotechnol 32:773CrossRefGoogle Scholar
  3. 3.
    Kang HW, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A (2016) A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol 34:312–319CrossRefGoogle Scholar
  4. 4.
    Ji S, Guvendiren M (2015) Recent advances in bioink design for 3D bioprinting of tissues and organs. Front Bioeng Biotechnol 5:23–31Google Scholar
  5. 5.
    Hölzl K, Lin S, Tytgat L, Van Vlierberghe S, Gu L, Ovsianikov A (2016) Bioink properties before, during and after 3D bioprinting. Biofabrication 8:32002CrossRefGoogle Scholar
  6. 6.
    Chimene D, Lennox KK, Kaunas RR, Gaharwar AK (2016) Advanced bioinks for 3D printing: a materials science perspective. Ann Biomed Eng 44:2090–2102CrossRefGoogle Scholar
  7. 7.
    Panwar A, Tan L (2016) Current status of bioinks for micro-extrusion-based 3D bioprinting. Molecules 21:E685CrossRefGoogle Scholar
  8. 8.
    Armstrong JPK, Burke M, Carter BM, Davis SA, Perriman AW (2016) 3D bioprinting using a templated porous bioink. Adv Healthc Mater 5:1724CrossRefGoogle Scholar
  9. 9.
    Axpe E, Oyen ML (2016) Applications of alginate-based bioinks in 3D bioprinting. Int J Mol Sci 17:1976CrossRefGoogle Scholar
  10. 10.
    Xu T, Zhao W, Zhu JM, Albanna MZ, Yoo JJ, Atala A (2013) Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology. Biomaterials 34:130–139CrossRefGoogle Scholar
  11. 11.
    Xu T, Baicu C, Aho M, Zile M, Boland T (2009) Fabrication and characterization of bio-engineered cardiac pseudo tissues. Biofabrication 1:35001CrossRefGoogle Scholar
  12. 12.
    Kosik-Kozioł A, Costantini M, Bolek T, Szoke K, Barbetta A, Brinchmann JE, Święszkowski W (2017) PLA short sub-micron fibers reinforcement of 3D bioprinted alginate constructs for cartilage regeneration. Biofabrication 9:044105CrossRefGoogle Scholar
  13. 13.
    Yeo MG, Lee JS, Chun W, Kim GH (2016) An innovative collagen-based cell-printing method for obtaining human adipose stem cell-laden structures consisting of core-sheath structures for tissue engineering. Biomacromolecules 17:1365–1375CrossRefGoogle Scholar
  14. 14.
    Nishiyama Y, Nakamura M, Henmi C, Yamaguchi K, Mochizuki S, Nakagawa H, Takiura K (2007) Fabrication of 3D cell supporting structures with multi-materials using the bio-printer. In: ASME 2007 International manufacturing science and engineering conference, american society of mechanical engineers, New York CityGoogle Scholar
  15. 15.
    Khalil S, Sun W (2009) Bioprinting endothelial cells with alginate for 3D tissue constructs. J Biomech Eng 131:111002CrossRefGoogle Scholar
  16. 16.
    Diogo GS, Gaspar VM, Serra IR, Fradique R, Correia IJ (2014) Manufacture of β- TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering. Biofabrication 6:25001CrossRefGoogle Scholar
  17. 17.
    Costantini M, Idaszek J, Szöke K, Jaroszewicz J, Dentini M, Barbetta A, Brinchmann JE, Święszkowski W (2016) 3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation. Biofabrication 8:35002CrossRefGoogle Scholar
  18. 18.
    Costantini M, Testa S, Mozetic P, Barbetta A, Fuoco C, Fornetti E, Tamiro F, Bernardini S, Jaroszewicz J, Święszkowski W, Trombetta M, Castagnoli L, Seliktar D, Garstecki P, Cesareni G, Cannata S, Rainer A, Gargioli C (2017) Biomaterials 131:98–110CrossRefGoogle Scholar
  19. 19.
    Colosi C, Costantini M, Latini R, Ciccarelli S, Stampella A, Barbetta A, Massimi M, Devirgiliis LC, Dentini M (2014) Rapid prototyping of chitosan-coated alginate scaffolds through the use of a 3D fiber deposition technique. J Mater Chem B 2:6779–6791CrossRefGoogle Scholar
  20. 20.
    Li Y, Liu Y, Jiang C, Li S, Liang G, Hu Q (2016) A reactor-like spinneret used in 3D printing alginate hollow fiber: a numerical study of morphological evolution. Soft Matter 12:2392–2399CrossRefGoogle Scholar
  21. 21.
    Costantini M, Colosi C, Święszkowski W, Barbetta A (2018) Co-axial wet-spinning in 3D bioprinting: state of the art and future perspective of microfluidic integration. Biofabrication 11:012001CrossRefGoogle Scholar
  22. 22.
    Cruise GM, Scharp DS, Hubbell JA (1998) Characterization of permeability and network structure of interfacially photopolymerized poly(ethylene glycol) diacrylate hydrogels. Biomaterials 14:1287CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2021

Authors and Affiliations

  • Marco Costantini
    • 1
  • Andrea Barbetta
    • 2
  • Wojciech Swieszkowski
    • 3
  • Dror Seliktar
    • 4
  • Cesare Gargioli
    • 5
  • Alberto Rainer
    • 6
    Email author
  1. 1.Institute of Physical Chemistry, Polish Academy of SciencesWarsawPoland
  2. 2.Department of ChemistrySapienza University of RomeRomeItaly
  3. 3.Warsaw University of TechnologyWarsawPoland
  4. 4.Faculty of Biomedical EngineeringTECHNION Israel Institute of TechnologyHaifaIsrael
  5. 5.University of Rome “Tor Vergata”RomeItaly
  6. 6.Università Campus Bio-Medico di RomaRomeItaly

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