3D Bioprinting pp 251-258 | Cite as

Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling

  • Jeremy M. CrookEmail author
  • Eva Tomaskovic-CrookEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2140)


Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter  10) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.

Key words

3D bioprinting Clinically amenable bioink Human induced pluripotent stem cells Multilineage Tissue 



The authors wish to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).


  1. 1.
    Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145–1147CrossRefGoogle Scholar
  2. 2.
    Crook JM, Peura T, Kravets L, Bosman A, Buzzard JJ, Horne R et al (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1:490–494CrossRefGoogle Scholar
  3. 3.
    Takahashi K, Tanabe K, Ohnuki M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131(5):861–872CrossRefGoogle Scholar
  4. 4.
    Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858):1917–1920CrossRefGoogle Scholar
  5. 5.
    Gu Q, Tomaskovic-Crook E, Wallace GG, Crook JM (2017) 3D bioprinting human induced pluripotent stem cell constructs for in situ cell proliferation and successive multi-lineage differentiation. Adv Healthc Mater 6:1700175CrossRefGoogle Scholar
  6. 6.
    Gu Q, Tomaskovic-Crook E, Lozano R, Chen Y, Kapsa RM, Zhou Q et al (2016) Functional 3D neural mini-tissues from printed gel-based human neural stem cells. Adv Healthc Mater 5:1429–1438CrossRefGoogle Scholar
  7. 7.
    Tomaskovic-Crook E, Zhang P, Ahtiainen A, Kaisvuo H, Lee CY, Beirne S et al (2019) Human neural tissues from neural stem cells using conductive biogel and printed polymer microelectrode arrays for 3D electrical stimulation. Adv Healthc Mater 8(15):1900425CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM FacilityUniversity of WollongongWollongongAustralia
  2. 2.Illawarra Health and Medical Research InstituteUniversity of WollongongWollongongAustralia
  3. 3.Department of Surgery, St Vincent’s HospitalThe University of MelbourneFitzroyAustralia

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