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3D Bioprinting pp 243-249 | Cite as

Bioprinting Strategies for Secretory Epithelial Organoids

  • Ganokon Urkasemsin
  • Sasitorn Rungarunlert
  • João N. FerreiraEmail author
Protocol
  • 59 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2140)

Abstract

Novel three-dimensional (3D) biofabrication platforms can allow magnetic 3D bioprinting (M3DB) by using magnetic nanoparticles to tag cells and then spatially arrange them in 3D around magnet dots. Here, we report an M3DB methodology to generate salivary gland-like epithelial organoids from stem cells. These organoids possess a neuronal network that responds to saliva neurostimulants.

Key words

Bioprinting Magnetic nanoparticles Organoids Salivary gland Xerostomia 

Notes

Acknowledgments

The development of this protocol and work was supported by Special Task Force for Activating Research (STAR) for Exocrine Gland Biology and Regeneration Research Group, Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University [grant number: STF 6202432001-1] and Chulalongkorn University Faculty of Dentistry [grant number: DRF62018] in Thailand to J.N.F. and by funding from Mahidol University [grant numbers: E04/2560, 83/2561, and 213/2562] in Thailand to S.R.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Ganokon Urkasemsin
    • 1
  • Sasitorn Rungarunlert
    • 1
  • João N. Ferreira
    • 2
    • 3
    Email author
  1. 1.Department of Preclinical and Applied Animal Science, Faculty of Veterinary ScienceMahidol UniversityNakhon PathomThailand
  2. 2.Exocrine Gland Biology and Regeneration Research Group, Faculty of DentistryChulalongkorn UniversityBangkokThailand
  3. 3.Faculty of DentistryNational University of SingaporeSingaporeSingapore

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