Bioprinting Strategies for Secretory Epithelial Organoids
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Novel three-dimensional (3D) biofabrication platforms can allow magnetic 3D bioprinting (M3DB) by using magnetic nanoparticles to tag cells and then spatially arrange them in 3D around magnet dots. Here, we report an M3DB methodology to generate salivary gland-like epithelial organoids from stem cells. These organoids possess a neuronal network that responds to saliva neurostimulants.
Key wordsBioprinting Magnetic nanoparticles Organoids Salivary gland Xerostomia
The development of this protocol and work was supported by Special Task Force for Activating Research (STAR) for Exocrine Gland Biology and Regeneration Research Group, Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University [grant number: STF 6202432001-1] and Chulalongkorn University Faculty of Dentistry [grant number: DRF62018] in Thailand to J.N.F. and by funding from Mahidol University [grant numbers: E04/2560, 83/2561, and 213/2562] in Thailand to S.R.
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