3D Bioprinting pp 199-215 | Cite as

Bioprinting for Human Respiratory and Gastrointestinal In Vitro Models

  • Manuela Estermann
  • Christoph Bisig
  • Dedy Septiadi
  • Alke Petri-Fink
  • Barbara Rothen-RutishauserEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2140)


Increasing ethical and biological concerns require a paradigm shift toward animal-free testing strategies for drug testing and hazard assessments. To this end, the application of bioprinting technology in the field of biomedicine is driving a rapid progress in tissue engineering. In particular, standardized and reproducible in vitro models produced by three-dimensional (3D) bioprinting technique represent a possible alternative to animal models, enabling in vitro studies relevant to in vivo conditions. The innovative approach of 3D bioprinting allows a spatially controlled deposition of cells and biomaterial in a layer-by-layer fashion providing a platform for engineering reproducible models. However, despite the promising and revolutionizing character of 3D bioprinting technology, standardized protocols providing detailed instructions are lacking. Here, we provide a protocol for the automatized printing of simple alveolar, bronchial, and intestine epithelial cell layers as the basis for more complex respiratory and gastrointestinal tissue models. Such systems will be useful for high-throughput toxicity screening and drug efficacy evaluation.

Key words

In vitro cultures Alveolar epithelial cells Bronchial epithelial cells Intestine epithelial cells Bioprinting technique 



This work was supported by the Swiss National Science Foundation (grant number CRSII5_171037), the Run4Science grant, and the Adolphe Merkle Foundation.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Manuela Estermann
    • 1
  • Christoph Bisig
    • 1
    • 2
  • Dedy Septiadi
    • 1
  • Alke Petri-Fink
    • 1
  • Barbara Rothen-Rutishauser
    • 1
    Email author
  1. 1.Adolphe Merkle InstituteUniversity of FribourgFribourgSwitzerland
  2. 2.Comprehensive Molecular AnalyticsHelmholtz Center MunichMunichGermany

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