Abstract
With the development of new materials and technologies, it is possible to access gene function and drug metabolism using a three-dimensional (3D) cell culture system, which is more suitable for mimicking the in vivo microenvironment of cultured tumor cells ex vivo. SeedEZ is a novel and versatile tool that allows culturing of different types of cells with user convenience and in a desired sequence. This system provides a bridge between traditional 2D culture and animal experiments. Here, we provide two examples demonstrating how to evaluate cancer cell growth by the SeedEZ 3D scaffold and cancer cell invasion by the SeedEZ 3D ring, in order to promote understanding of the necessity of this novel cell culture system.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Duval K, Grover H, Han L-H, Mou Y, Pegoraro AF, Fredberg J et al (2017) Modeling physiological events in 2D vs. 3D cell culture. Physiology 32:266–777
Yamada KM, Cukierman E (2007) Modeling tissue morphogenesis and cancer in 3D. Cell 130:601–610
Lv D, Hu Z, Lu L, Lu H, Xu X (2017) Three-dimensional cell culture: a powerful tool in tumor research and drug discovery. Oncol Lett 14:6999–7010
Fang Y, Eglen RM (2017) Three-dimensional cell cultures in drug discovery and development. SLAS Discov 22(5):456–472
Jin G, Xu C, Zhang X, Long J, Rezaeian AH, Liu C et al (2018) Atad3a suppresses Pink1-dependent mitophagy to maintain homeostasis of hematopoietic progenitor cells. Nat Immunol 19(1):29–40
Xiong Y, He L, Shay C, Lang L, Loveless J, Yu J et al (2019) Nck-associated protein 1 associates with HSP90 to drive metastasis in human non-small-cell lung cancer. J Exp Clin Cancer Res 38(1):122. https://doi.org/10.1186/s13046-019-1124-0
Lee BKB, Gan CP, Chang JK, Tan JL, Fadlullah MZ, Abdul Rahman ZA et al (2018) GENIPAC: a genomic information portal for head and neck cancer cell systems. J Dent Res 97(8):909–916
Carney DN, Gazdar AF, Nau M, Minna JD (1985) Biological heterogeneity of small cell lung cancer. Semin Oncol 12(3):289–303
Teng Y, Ren X, Li H, Shull A, Kim J, Cowell JK (2016) Mitochondrial ATAD3A combines with GRP78 to regulate the WASF3 metastasis-promoting protein. Oncogene 35(3):333–343
Dickerson T, Jauregui CE, Teng Y (2017) Friend or foe? Mitochondria as a pharmacological target in cancer treatment. Future Med Chem 9(18):2197–2210
Teng Y, Qin H, Bahassan A, Bendzunas NG, Kennedy EJ, Cowell JK (2016) The WASF3-NCKAP1-CYFIP1 complex is essential for breast cancer metastasis. Cancer Res 76(17):5133–5142
Acknowledgments
This research was supported by NIH grant R03DE028387 and R01DE028351 (to Y.T.).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Shay, C., Lang, L., Teng, Y. (2020). Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System. In: Guest, P. (eds) Clinical and Preclinical Models for Maximizing Healthspan. Methods in Molecular Biology, vol 2138. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0471-7_9
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0471-7_9
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0470-0
Online ISBN: 978-1-0716-0471-7
eBook Packages: Springer Protocols