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Rat Models of Virus-Induced Type 1 Diabetes

  • James C. Needell
  • Danny ZiprisEmail author
Protocol
  • 65 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2128)

Abstract

Studies performed in humans and animal models have implicated the environment in the etiology of type 1 diabetes (T1D), but the nature and timing of the interactions triggering β cell autoimmunity are poorly understood. Virus infections have been postulated to be involved in disease mechanisms, but the underlying mechanisms are not known. It is exceedingly difficult to establish a cause-and-effect relationship between viral infection and diabetes in humans. Thus, we have used the BioBreeding Diabetes-Resistant (BBDR) and the LEW1.WR1 rat models of virus-induced disease to elucidate how virus infection leads to T1D. The immunophenotype of these strains is normal, and spontaneous diabetes does not occur in a specific pathogen-free environment. However, β cell inflammation and diabetes with many similarities to the human disease are induced by infection with the parvovirus Kilham rat virus (KRV). KRV-induced diabetes in the BBDR and LEW1.WR1 rat models is limited to young animals and can be induced in both male and female rats. Thus, these animals provide a powerful experimental tool to identify mechanisms underlying virus-induced T1D development.

Key words

BioBreeding Diabetes-Resistant (BBDR) rat Chang human liver cell line H-1 parvovirus Kilham rat virus LEW1.WR1 rat Normal rat kidney (NRK) cell line 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Innate Biotechnologies LLCDenverUSA

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