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Using Pancreas Tissue Slices for the Study of Islet Physiology

  • Julia K. Panzer
  • Christian M. Cohrs
  • Stephan SpeierEmail author
Protocol
  • 63 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2128)

Abstract

Studies on islet of Langerhans physiology are crucial to understand the role of the endocrine pancreas in diabetes pathogenesis and the development of new therapeutic approaches. However, so far most research addressing islet of Langerhans biology relies on islets obtained via enzymatic isolation from the pancreas, which is known to cause mechanical and chemical stress, thus having a major impact on islet cell physiology. To circumvent the limitations of islet isolation, we have pioneered a platform for the study of islet physiology using the pancreas tissue slice technique. This approach allows to explore the detailed three-dimensional morphology of intact pancreatic tissue at a cellular level and to investigate islet cell function under near-physiological conditions. The described procedure is less damaging and faster than alternative approaches and particularly advantageous for studying infiltrated and structurally damaged islets. Furthermore, pancreas tissue slices have proven valuable for acute studies of endocrine as well as exocrine cell physiology in their conserved natural environment. We here provide a detailed protocol for the preparation of mouse pancreas tissue slices, the assessment of slice viability, and the study of pancreas cell physiology by hormone secretion and immunofluorescence staining.

Key words

Tissue slices In situ islet cell function Insulin secretion Immunofluorescence staining Live/dead staining 

Notes

Acknowledgment

This work was supported with funds from the Paul Langerhans Institute Dresden (PLID) of Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, the German Ministry of Education and Research (BMBF) to the German Centre for Diabetes Research (DZD), the DFG SFB/Transregio 127, the European Foundation for the Study of Diabetes (EFSD)/Boehringer Ingelheim Basic Research Programme, and the Helmsley Charitable Trust George S. Eisenbarth nPOD Award for Team Science.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Julia K. Panzer
    • 1
    • 2
    • 3
  • Christian M. Cohrs
    • 1
    • 2
    • 3
  • Stephan Speier
    • 1
    • 2
    • 3
    Email author
  1. 1.Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum MünchenNeuherbergGermany
  2. 2.Institute of Physiology, Faculty of Medicine, Technische Universität DresdenDresdenGermany
  3. 3.German Center for Diabetes Research (DZD)München-NeuherbergGermany

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