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Immunoblotting in Detection of Tumor-Associated Antigens in Esophageal Squamous Cell Carcinoma

  • Farhadul Islam
  • Vinod Gopalan
  • Alfred K. LamEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2129)

Abstract

Tumor-associated antigens (TAAs) can be used as cancer markers and as signposts of therapeutic targets since their inimitable expression in cancer or significant overexpression in esophageal squamous cell carcinoma (ESCC) correlates with the initiation and progression of the diseases. Immunoblotting, also known as Western blotting or protein blotting, is a core technique in cell and molecular biology to detect proteins and glycoproteins. The technique allows detection of TAAs from complex protein samples such as in serum, aspirate, or solid tumor homogenate. In the process, proteins are separated according to the molecular weight. They were visualized within a gel matrix and then transferred to a supporting membrane. Finally, they are probed for binding with corresponding antibodies and identified the target proteins. Herein, we describe the Western blots analysis to detect protein or glycoprotein in samples from patients with esophageal squamous cell carcinoma (ESCC) or cells derived from ESCC.

Key words

Tumor-associated antigen Immunoblots Western blots Protein blots ESCC 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Farhadul Islam
    • 1
    • 2
  • Vinod Gopalan
    • 1
  • Alfred K. Lam
    • 3
    Email author
  1. 1.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of RajshahiRajshahiBangladesh
  3. 3.Cancer Molecular Pathology, School of MedicineGriffith UniversityGold CoastAustralia

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