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Energy Transfer Systems for In Vivo Tracking

  • Raul Neri
  • Asanka Sajini Yapa
  • Stefan H. BossmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

With recent advances, fluorescent imaging has gained momentum as an important tool for in vivo imaging. FRET systems consist of molecules that absorb in the near-infrared region which are efficient candidates for in vivo imaging, basic research, and clinical applications. Nontoxic, photostable fluorophores, such as fluorescent proteins and dyes, can successfully be used to visualize spatial and temporal dynamics of living cells. Selected cells to be injected are first tagged with the FRET-based biosensor and then injected to the living animal. Then, these foreign cells in the host body can be visualized under fluorescence microscope via excitation of the fluorophores at the correct wavelength.

Key words

Fluorescence Endothelial cell tracking Förster resonance energy transfer (FRET) Semi conducting π-conjugated polymers (SPs) Critical limb ischemia (CLI) Suzuki polymerization Semi conducting π-conjugated polymer based nanoparticles (SPNs) 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Raul Neri
    • 1
  • Asanka Sajini Yapa
    • 1
  • Stefan H. Bossmann
    • 2
    Email author
  1. 1.Department of Chemistry and Johnson Cancer Research CenterKansas State UniversityManhattanUSA
  2. 2.Department of Chemistry and Johnson Cancer CenterKansas State UniversityManhattanUSA

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