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Cell Tracking pp 107-116 | Cite as

MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles

  • Wencheng Zhu
  • Ye Xu
  • Rongrong Jin
  • Changqiang Wu
  • Hua AiEmail author
Protocol
  • 104 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)

Abstract

Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.

Key words

Cell labeling Cell migration Magnetic resonance imaging Superparamagnetic iron oxide Dendritic cell 

Notes

Acknowledgement

We gratefully acknowledge financial support from the Funds for Creative Research Groups of China (81621003) and National Key Basic Research Program of China (2013CB933903).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Wencheng Zhu
    • 1
    • 2
  • Ye Xu
    • 1
  • Rongrong Jin
    • 1
  • Changqiang Wu
    • 1
  • Hua Ai
    • 1
    • 3
    Email author
  1. 1.National Engineering Research Center for BiomaterialsSichuan UniversityChengduChina
  2. 2.Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghaiChina
  3. 3.Department of Radiology, West China HospitalSichuan UniversityChengduChina

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