Cell Tracking pp 107-116 | Cite as

MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles

  • Wencheng Zhu
  • Ye Xu
  • Rongrong Jin
  • Changqiang Wu
  • Hua AiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2126)


Cell tracking via MRI has drawn much attention recently for its sensitive, deep, and real-time properties and high spatial resolution. In a previous chapter, the labeling and tracking of superparamagnetic iron oxide (SPIO)-nanoparticle-loaded stem cells have been well summarized (Sykova et al., Methods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.

Key words

Cell labeling Cell migration Magnetic resonance imaging Superparamagnetic iron oxide Dendritic cell 



We gratefully acknowledge financial support from the Funds for Creative Research Groups of China (81621003) and National Key Basic Research Program of China (2013CB933903).


  1. 1.
    Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021CrossRefGoogle Scholar
  2. 2.
    Jin RR, Lin BB, Li DY et al (2014) Superparamagnetic iron oxide nanoparticles for MR imaging and therapy: design considerations and clinical applications. Curr Opin Pharmacol 18:18–27CrossRefGoogle Scholar
  3. 3.
    Kircher MF, Gambhir SS, Grimm J (2011) Noninvasive cell-tracking methods. Nat Rev Clin Oncol 8:677–688CrossRefGoogle Scholar
  4. 4.
    Bulte JW (2009) In vivo MRI cell tracking: clinical studies. Am J Roentgenol 193:314–325CrossRefGoogle Scholar
  5. 5.
    Li L, Jiang W, Luo K et al (2013) Superparamagnetic iron oxide nanoparticles as MRI contrast agents for non-invasive stem cell labeling and tracking. Theranostics 3:595–615CrossRefGoogle Scholar
  6. 6.
    Rogers WJ, Meyer CH, Kramer CM (2006) Technology insight: in vivo cell tracking by use of MRI. Nat Rev Cardiol 3:554CrossRefGoogle Scholar
  7. 7.
    Wang Z, Liu G, Sun J et al (2012) N-alkyl-polyethylenimine stabilized iron oxide nanoparticles as MRI visible transfection agents. J Nanosci Nanotechnol 12:879–886CrossRefGoogle Scholar
  8. 8.
    Liu G, Wang ZY, Lu J et al (2011) Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging. Biomaterials 32:528–537CrossRefGoogle Scholar
  9. 9.
    Liu G, Xia CC, Wang ZY et al (2011) Magnetic resonance imaging probes for labeling of chondrocyte cells. J Mater Sci Mater Med 22:601–606CrossRefGoogle Scholar
  10. 10.
    Xu Y, Wu CQ, Zhu WC et al (2015) Superparamagnetic MRI probes for in vivo tracking of dendritic cell migration with a clinical 3 T scanner. Biomaterials 58:63–71CrossRefGoogle Scholar
  11. 11.
    Chen C, Yu H, Xia R et al (2014) Magnetic resonance tracking of endothelial progenitor cells labeled with alkyl-polyethylenimine 2 kDa/superparamagnetic iron oxide in a mouse lung carcinoma xenograft model. Mol Imaging 13:1–11CrossRefGoogle Scholar
  12. 12.
    Wu CQ, Xu Y, Yang L et al (2015) Negatively charged magnetite nanoparticle clusters as efficient MRI probes for dendritic cell labeling and in vivo tracking. Adv Funct Mater 25:3581–3591CrossRefGoogle Scholar
  13. 13.
    Riemer J, Hoepken HH, Czerwinska H et al (2004) Colorimetric ferrozine-based assay for the quantitation of iron in cultured cells. Anal Biochem 331:370–375CrossRefGoogle Scholar
  14. 14.
    Sykova E, Jendelova P, Herynek V (2011) Magnetic resonance imaging of stem cell migration. Methods Mol Biol 750:79–90CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Wencheng Zhu
    • 1
    • 2
  • Ye Xu
    • 1
  • Rongrong Jin
    • 1
  • Changqiang Wu
    • 1
  • Hua Ai
    • 1
    • 3
    Email author
  1. 1.National Engineering Research Center for BiomaterialsSichuan UniversityChengduChina
  2. 2.Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghaiChina
  3. 3.Department of Radiology, West China HospitalSichuan UniversityChengduChina

Personalised recommendations