Avian Influenza Virus RNA Extraction

  • Erica SpackmanEmail author
  • Scott A. Lee
Part of the Methods in Molecular Biology book series (MIMB, volume 2123)


The efficient extraction and purification of viral RNA is critical for downstream molecular applications such as the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, gene sequencing, or quantification of avian influenza (AI) virus by molecular methods from experimentally infected birds. Samples can generally be divided into two types: enriched (e.g., virus stocks) and non-enriched (e.g., clinical). Clinical samples, which may be tissues or swab material, are the most difficult to process due to the complex sample composition and possibly low virus titers. In this chapter, two well-established procedures for the extraction of AI virus RNA from common clinical specimen types and enriched virus stocks will be presented.

Key words

RNA extraction Influenza nucleic acid Specimen processing Swab material Avian influenza 


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Copyright information

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. 2020

Authors and Affiliations

  1. 1.Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research CenterUS Department of Agriculture, Agricultural Research ServiceAthensUSA

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