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Protocols for Studying Murine ILC Development

  • Matthew T. Stier
  • R. Stokes PeeblesJrEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2121)

Abstract

Understanding the origins and developmental trajectory of innate lymphoid cell (ILC) progenitors has been of substantial interest to the fields of ILC biology and immunology. While mature ILC are rare lymphocytes, ILC progenitors represent an even smaller fraction of cells, providing additional challenges in studying them. Moreover, though the approaches to studying these cells are conceptually straightforward, the technical nuances that underlie them can substantially affect the quality of the data. Herein, we provide a detailed protocol for assessing the frequency of ILC progenitors in the bone marrow, their phenotype, and their potential to develop into mature ILC. These methods make up the foundation of in vivo investigations into ILC development, and we hope these thorough protocols and associated notes facilitate additional, high-quality inquiries into this fascinating field.

Key words

Innate lymphoid cells (ILC) Natural killer (NK) cells Common lymphoid progenitors Flow cytometry Adoptive transfer 

Notes

Acknowledgments

Optimization of the protocols described herein was supported by the National Institutes of Health (R01AI145265, R01AI124456, R01AI111820, R21AI145397, and U19AI095227) and the US Department of Veterans Affairs (I01BX004299).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Division of Allergy, Pulmonary and Critical Care Medicine, Department of MedicineVanderbilt University Medical CenterNashvilleUSA

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