Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment
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Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment.
Key wordsB16 Melanoma ILCs PD-1
We would like to thank the Academy of Medical Sciences, Springboard Award [SBF003\1129] supported by Wellcome Trust and Newcastle University Research Fellowship for supporting this work. G.M. is supported by a Medical Research Council [MRC] Discovery Medicine North Ph.D. studentship. M.P. is supported by a Wellcome Trust Vacation Scholarship [216503/Z/19/Z].