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Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

  • Grace Mallett
  • Warren Patterson
  • Megan Payne
  • Shoba AmarnathEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2121)

Abstract

Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment.

Key words

B16 Melanoma ILCs PD-1 

Notes

Acknowledgments

We would like to thank the Academy of Medical Sciences, Springboard Award [SBF003\1129] supported by Wellcome Trust and Newcastle University Research Fellowship for supporting this work. G.M. is supported by a Medical Research Council [MRC] Discovery Medicine North Ph.D. studentship. M.P. is supported by a Wellcome Trust Vacation Scholarship [216503/Z/19/Z].

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Grace Mallett
    • 1
  • Warren Patterson
    • 1
  • Megan Payne
    • 1
  • Shoba Amarnath
    • 1
    Email author
  1. 1.Laboratory of T cell Regulation, Institute of Cellular MedicineNewcastle UniversityNewcastle upon TyneUK

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