Abstract
Innate lymphoid cells (ILCs) are important regulators of the early responses to infection at mucosal barriers, including the intestine. Recently, we have shown that specific ILC3 subsets protect against enteric bacterial pathogens. Here, we describe a mouse model of oral infection by Yersinia enterocolitica (Y. enterocolitica) and several different methodologies to assess the severity of the infection. We also detail how ILC3 subsets can be isolated from the mouse small intestine and transferred into recipient immune deficient mice to study the function of these ILCs in the small intestine.
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References
Artis D, Spits H (2015) The biology of innate lymphoid cells. Nature 517:293–230
Klose CS, Kiss EA, Schwierzeck V et al (2013) A T-bet gradient controls the fate and function of CCR6-RORgammat+ innate lymphoid cells. Nature 494:261–265
Luci C, Reynders A, Ivanov I et al (2009) Influence of the transcription factor RORgammat on the development of NKp46+ cell populations in gut and skin. Nat Immunol 10:75–82
Sanos SL, Bui VL, Mortha A et al (2009) RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat Immunol 10:83–91
Satoh-Takayama N, Vosshenrich CA, Lesjean-Pottier S et al (2008) Microbial flora drives interleukin 22 production in intestinal NKp46+ cells that provide innate mucosal immune defense. Immunity 29:958–970
Eberl G, Marmon S, Sunshine MJ et al (2004) An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol 5:64–73
Cella M, Fuchs A, Vermi W et al (2009) A human natural killer cell subset provides an innate source of IL-22 for mucosal immunity. Nature 457:722–725
Sonnenberg GF, Monticelli LA, Elloso MM et al (2011) CD4(+) lymphoid tissue-inducer cells promote innate immunity in the gut. Immunity 34:122–134
Buonocore S, Ahern PP, Uhlig HH et al (2010) Innate lymphoid cells drive interleukin-23-dependent innate intestinal pathology. Nature 464:1371–1375
Goto Y, Obata T, Kunisawa J et al (2014) Innate lymphoid cells regulate intestinal epithelial cell glycosylation. Science 345:1254009
Song C, Lee JS, Gilfillan S et al (2015) Unique and redundant functions of NKp46+ ILC3s in models of intestinal inflammation. J Exp Med 212:1869–1882
Trulzsch K, Oellerich MF, Heesemann J (2007) Invasion and dissemination of Yersinia enterocolitica in the mouse infection model. Adv Exp Med Biol 603:279–285
Bottone EJ (1997) Yersinia enterocolitica: the charisma continues. Clin Microbiol Rev 10:257–276
Cover TL, Aber RC (1989) Yersinia enterocolitica. N Engl J Med 321:16–24
Seo GY, Shui JW, Takahashi D et al (2018) LIGHT-HVEM signaling in innate lymphoid cell subsets protects against enteric bacterial infection. Cell Host Microbe 24:249–260.e244
Trulzsch K, Sporleder T, Igwe EI et al (2004) Contribution of the major secreted yops of Yersinia enterocolitica O:8 to pathogenicity in the mouse infection model. Infect Immun 72:5227–5234
Guo X, Muite K, Wroblewska J et al (2016) Purification and adoptive transfer of group 3 gut innate lymphoid cells. Methods Mol Biol 1422:189–196
Acknowledgments
This work was supported by grants from the National Institutes of Health P01 DK46763, R01 AI61516, and MIST U01 AI125955 to M.K.; F32 AI140581 to D.A.G.; and National Research Foundation (NRF) of Korea 2013R1A1A2057931 to G.-Y.S. We thank the staff of the Microscopy & Histology Core (Zbigniew Mikulski and Angela Denn), Flow Cytometry Core and the Department of Laboratory Animal Care (DLAC) at LJI for excellent technical assistance, SDx Histopathology Inc., (Carlsbad, CA) for help with histology analysis.
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Seo, GY., Giles, D.A., Kronenberg, M. (2020). Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets. In: Amarnath, S. (eds) Innate Lymphoid Cells . Methods in Molecular Biology, vol 2121. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0338-3_12
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DOI: https://doi.org/10.1007/978-1-0716-0338-3_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0337-6
Online ISBN: 978-1-0716-0338-3
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