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Group 2 ILC Functional Assays in Allergic Airway Inflammation

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Part of the Methods in Molecular Biology book series (MIMB, volume 2121)

Abstract

ILC2s are a rare innate cell population capable of rapidly producing type 2 cytokines prior to the recruitment and expansion of adaptive type 2 T helper cells. As a result, they are implicated in the pathogenesis of many type-2 immune-mediated diseases, including allergic airway inflammation. Here we describe methods for interrogating and analyzing ILC2 biology in the context of allergic airway inflammation, such as flow cytometric analysis of mouse and human ILC2s as well as live imaging of pulmonary ILC2s.

Key words

ILC2 Asthma Allergic airway inflammation Lung Flow cytometry Microscopy Imaging 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Inflammation, Repair & Development, National Heart & Lung InstituteImperial College LondonLondonUK

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