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Development of Infectious Clone of Virus

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 49 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

An infectious clone is a full-length DNA clone from which infectious transcripts can be obtained in vitro or in vivo with a suitable promoter. An infectious clone is the basic need to study functional genomics, replication and expression of viral proteins and in understanding host–virus interactions. Infectious clones, considered as the pools of viral genes for designing antiviral strategies, are an essential source of material for the preparation of new viral vectors. In this chapter, production of in vitro and in vivo clones of viruses is discussed.

Key words

Full-length infectious clone Full-length infectious cDNA In vitro transcription In vivo transcription Bacteriophage RNA polymerase promoter Agro-inoculation Cauliflower mosaic virus 35S promoter 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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