Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Part of the Springer Protocols Handbooks book series (SPH)


All single-stranded RNA (ssRNA) virus-infected plants produce double-stranded RNA (dsRNA) during their replication cycle. dsRNAs are more stable than its counterpart ssRNA. Many protocols are available for the isolation of dsRNA from virus-infected plants that are used as template for cDNA synthesis followed by PCR assay. In this chapter, suitable protocol for the isolation of ds RNA from plant sources is discussed.

Key words

Replicative form of virus RNA template from plant CF-11 column 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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