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Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 44 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

All single-stranded RNA (ssRNA) virus-infected plants produce double-stranded RNA (dsRNA) during their replication cycle. dsRNAs are more stable than its counterpart ssRNA. Many protocols are available for the isolation of dsRNA from virus-infected plants that are used as template for cDNA synthesis followed by PCR assay. In this chapter, suitable protocol for the isolation of ds RNA from plant sources is discussed.

Key words

Replicative form of virus RNA template from plant CF-11 column 

References

  1. Dodds JA, Morris TJ, Jordan RL (1984) Plant viral double stranded RNA. Annu Rev Phytopathol 22:151–168CrossRefGoogle Scholar
  2. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus infected plant and fungal tissue. Phytopathology 69:854–858CrossRefGoogle Scholar
  3. Morris TJ, Dodds JA, Hillman B, Jordon R, Lommel SA, Tamaki SJ (1983) Viral specific dsRNA. Diagnostic value for plant virus disease identification. Plant Mol Biol Rep 1:27–30CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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