In order to amplify plant DNA viruses, either viral DNA isolated from purified viral preparations or total DNAs isolated from virus-infected plants are used as template. The quantity and quality of DNA extracted are critical for successful amplification. Isolating quality DNA from plant tissues/cells involves several challenges, and certain plant tissues pose difficulty in processing due to the presence of polysaccharides, phenols, pigments and various secondary plant metabolites. A protocol that works with one plant group may fail with others. Consequently, a number of DNA isolation methods have been developed for different target groups. Many protocols for the isolation of total DNA from different plant species have been reported, but no single method that is applicable for all plant species is available. In this chapter, two widely used methods of DNA isolation is discussed with suitable protocols for obtaining a good quality DNA.
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