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Agarose Gel Electrophoresis for Nucleic Acids

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 55 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Agarose gel electrophoresis is the easiest, most popular and effective way of separating and analysing nucleic acid fragments to assess the quality and quantity of DNA and RNA on the basis of charge by applying an electric field to the electrophoretic apparatus. The DNA and RNA can be visualized in the gel by the addition of ethidium bromide or other dyes under UV light. The use of agarose gel electrophoresis revolutionized the separation of nucleic acids of different sizes. The rate of migration of a nucleic acid molecule through a gel is determined by the size, agarose concentration, nucleic acid conformation, voltage applied and electrophoresis buffer. The different steps involved in the process of agarose gel electrophoresis are discussed in detail in this chapter.

Key words

Agarose Horizontal gel electrophoresis Integrity of nucleic acid Quality of nucleic acid 

References

  1. Sambrook J, Russel DW (2001) Molecular cloning, vol I–III, 3rd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  2. Simpson CF, Whittaker M (eds) (1983) Electrophoretic techniques. Academic Press, LondonGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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