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Electron Microscopy and Utramicrotomy

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 46 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Virus particles can be seen only in transmission electron microscope. Transmission electron microscope not only helps to assess the purity of virus preparation but also throws light on the shape, size and surface details of virus particles. It is used to measure the dimension of the virus particles. Observation of virus particle morphology through electron microscopy (EM) is carried out either with purified virus preparations or with crude extracts of infected plant sap after negative staining. Besides, electron microscopy is also used in serological diagnosis of viruses through immunosorbent electron microscopy (ISEM). In the present chapter, process of sample preparation for the observation of shape and size of the virus particles in electron microscope is discussed. The process of ultrathin sectioning of the infected tissues for the observation of the virus particles in situ through EM is also discussed.

Key words

Virus particle morphology Electron microscope Leaf dip method Negative stains Ultramicrotomy Fixation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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