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Storage and Preservation of Plant Virus Cultures

  • Alangar Ishwara Bhat
  • Govind Pratap Rao
Protocol
  • 51 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Viruses are obligate pathogens that cannot be cultured without living in host. Plant viruses require special preservation methods to ensure long-term viability/infectivity of the sample and to maintain the original characteristics. Usually symptomatic leaf tissue of virus-infected plants is taken and kept as original sample for future study purpose. Many different methods are employed for the storage of virus cultures like desiccation, freeze drying (lyophilization), freezing and low temperature storage in liquid nitrogen, which are discussed in this chapter.

Key words

Desiccator Freeze-drying Low-temperature storage Virus culture Liquid nitrogen Lyophilization 

References

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  5. McKinney HH, Silber G, Greeley LW (1965) Properties of virus in crude sap. Phytopathology 55:1043–1044Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Alangar Ishwara Bhat
    • 1
  • Govind Pratap Rao
    • 2
  1. 1.Division of Crop ProtectionICAR-Indian Institute of Spices ResearchKozhikodeIndia
  2. 2.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia

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