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RNA Interference and CRISPR Technologies pp 351–364Cite as

CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2115))

Abstract

A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.

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Authors and Affiliations

  1. Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO, USA

    Kasidet Hiranniramol, Yuhao Chen & Xiaowei Wang

Authors
  1. Kasidet Hiranniramol
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  2. Yuhao Chen
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  3. Xiaowei Wang
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Corresponding author

Correspondence to Xiaowei Wang .

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Editors and Affiliations

  1. Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

    Mouldy Sioud

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Hiranniramol, K., Chen, Y., Wang, X. (2020). CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity. In: Sioud, M. (eds) RNA Interference and CRISPR Technologies. Methods in Molecular Biology, vol 2115. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0290-4_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0290-4_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0289-8

  • Online ISBN: 978-1-0716-0290-4

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