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RNA Interference and CRISPR Technologies pp 327–349Cite as

Use of RNA Interference with TCR Transfer to Enhance Safety and Efficiency

Part of the Methods in Molecular Biology book series (MIMB,volume 2115)

Abstract

The gene transfer of T-cell receptors (TCRs) is an attractive strategy for adoptive cell therapy, allowing the transfer of reactivity against antigens that may not otherwise engender an immune response. The TCRs recognize intracellular or extracellular antigens presented in the context of MHC class I or II, respectively. This broadens the range of targets considerably, compared to antibodies and chimeric antigen receptors, that are generally confined to surface antigens. However, TCR transfer must overcome some technical hurdles, relating to interference with endogenous α- and β-TCR chains and competition with other existing TCR infrastructure of T cells. In this review, we will outline the challenges facing TCR gene transfer and compare several approaches to address them. We will then focus upon one of the most promising amongst these—RNA interference—and detail the methods involved in designing and using this technology.

Key words

  • T cell receptor
  • TCR gene transfer
  • RNA interference
  • Small interfering RNAs
  • immunotherapy
  • Adoptive cell therapy

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Authors and Affiliations

  1. Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

    Nicholas Paul Casey & Jon Amund Kyte

  2. Department of Personalized Cancer Immunotherapy, Mie University Graduate School of Medicine, Tsu, Mie, Japan

    Hiroshi Fujiwara

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  1. Nicholas Paul Casey
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  2. Jon Amund Kyte
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  3. Hiroshi Fujiwara
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Correspondence to Nicholas Paul Casey .

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  1. Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

    Mouldy Sioud

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Casey, N.P., Kyte, J.A., Fujiwara, H. (2020). Use of RNA Interference with TCR Transfer to Enhance Safety and Efficiency. In: Sioud, M. (eds) RNA Interference and CRISPR Technologies. Methods in Molecular Biology, vol 2115. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0290-4_18

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