Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species

  • Reka A. Haraszti
  • Joerg E. Braun
Part of the Methods in Molecular Biology book series (MIMB, volume 2113)


Colocalization single-molecule spectroscopy (CoSMoS) allows studying RNA-protein complexes in the full complexity of their cellular environment at single-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe an approach to monitor multiple RNA species simultaneously to enable direct comparison. This approach represents a technological development to avoid conventional inter-experiment comparisons.

Key words

RNA spectroscopy CoSMoS Colocalization single-molecule spectroscopy RNA-protein complex RNP TIRF microscopy Single-molecule spectroscopy Single-molecule fluorescence microscopy 



Joerg E. Braun acknowledges funding from the Human Frontier Science Program (HFSP) LT000166/2013 and the European Molecular Biology Organization (EMBO) ALTF 890-2012. Joerg E. Braun is supported by funding to the laboratory of Melissa J. Moore: NIH R01 GM053007.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Reka A. Haraszti
    • 1
  • Joerg E. Braun
    • 1
  1. 1.RNA Therapeutics InstituteUniversity of Massachusetts Medical SchoolWorcesterUSA

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