Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice

  • Jakub Tomala
  • Jamie B. SpanglerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2111)


Flow cytometry has revolutionized the field of molecular immunology, enabling the monitoring and characterization of immune events at the single-cell level. Here, we describe a flow cytometry-based workflow to quantify the activation of specific immune cell subsets in mice in response to a molecular intervention. Compared to laborious long-term disease models, this technique allows for relatively rapid evaluation of candidate therapeutics designed to elicit a targeted immune response. This approach has the range to address both disease applications in which an immunostimulatory effect would be desired (e.g., cancer, infectious disease) or those in which an immunosuppressive effect would be desired (e.g., autoimmune disorders, transplantation medicine). Overall, our technique presents a powerful and accessible strategy for preliminary in vivo assessment of potential immunotherapeutics.

Key words

Immunology T cell Natural killer cell Flow cytometry Immunophenotyping Immunotherapy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringJohns Hopkins UniversityBaltimoreUSA
  2. 2.Department of Chemical and Biomolecular EngineeringJohns Hopkins UniversityBaltimoreUSA
  3. 3.Institute of Microbiology of the Czech Academy of SciencesPragueCzech Republic

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